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IPTG

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제품명 IPTG
제조사 MP Biomedicals
제품번호 02194029
사이즈 100g

 

ISOPROPYL-β-D-THIOGALACTOPYRANOSIDE

Molecular Biology, ≥99%, White Powder

·        Download MSDS (PDF)

Synonyms IPTG, Isopropyl β-D-thiogalactoside, l-Propyl-b-D-thiogalactopyranoside

 

·        CAS Number 367-93-1

·        Molecular Formula C9H18O5S

·        Molecular Weight 238.3

·        Beilstein Registry Number 4631

·        EC Number 206-703-0

MDL Number MFCD00063273

 

Applications

Isopropyl b-D-1-thiogalactopyranoside is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal. It is commonly used in cloning procedures that require induction of β-galactosidase activity. It is also used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

 

Product Description

Isopropyl b-D-1-thiogalactopyranoside is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli. It functions by binding to the lac repressor and altering its conformation. This inactivation prevents the repression of the b-galactosidase coding lac gene.

 

Typical Working Concentration

The final concentration typically used in indicator plates is 0.2 mM.

 

 

 

 

 

 

 

 

 

 

 

 

 


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